citB [2017-8-11 16:0:3]

aconitase, trigger enzyme

Locus: BSU18000

Isoelectric point: 4.90

Molecular weight: 99.14 kDa

Protein length: 909 aa

Gene length: 2727 bp

Function: TCA cycle

Product: aconitase, trigger enzyme

Essential: no

E.C.: 4.2.1.3

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Carbon core metabolism → TCA cycle
• Metabolism → Amino acid/ nitrogen metabolism → Utilization of amino acids → Utilization of branched-chain amino acids
• Information processing → Regulation of gene expression → Trigger enzyme → Trigger enzyme that acts by binding of a specific RNA element
• Information processing → Regulation of gene expression → RNA binding regulators

Gene

Coordinates: 1,926,680 → 1,929,409

Phenotypes of a mutant

• glutamate auxotrophy and a defect in sporulation PubMed

The protein

Catalyzed reaction/ biological activity

• Citrate <=> isocitrate
• Binding to iron responsive elements (IRE RNA) in the absence of the FeS cluster PubMed

Cofactors

• FeS cluster

Structure

• 1L5J (E. coli) PubMed

Additional information

• B. subtilis aconitase is both an enzyme and an RNA binding protein (moonlighting protein) PubMed
• extensive information on the structure and enzymatic properties of CitB can be found at Proteopedia
• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: citB-yneN
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CodY: repression, PubMed, in CodY regulon
  • CcpC: repression, (molecular inducer: citrate) PubMed PubMed, in CcpC regulon
  • CcpA: repression, PubMed, in CcpA regulon

  Regulation

  • expressed upon transition into the stationary phase (AbrB) PubMed, indirect negative regulation by AbrB PubMed

Other regulations

• FsrA: translation repression, PubMed

Biological materials

Mutant

• GP1275 (ΔcitB::erm), available in Jörg Stülke's lab
• GP1441 (ΔcitB::spc), available in Jörg Stülke's lab
• 1A999 (citB::spec), [Pubmed| ], available at BGSC
• GP2338 (ΔcitB::kan), Cre-recombinase is integrated in sacA, available in Jörg Stülke's lab
• GP2339 (ΔcitB::lox72), Cre-recombinase is integrated in sacA, available in Jörg Stülke's lab

LacZ fusion

• pGP700 (in pAC5), available in Jörg Stülke's lab

GFP fusion

• GP1434 (spc, based on pGP1870), available in Jörg Stülke's lab

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

FLAG-tag construct

• GP1144 (spc, based on pGP1331), available in Jörg Stülke's lab
• GP1145 (kan), available in Jörg Stülke's lab

Antibody

• available in Linc Sonenshein's lab

Labs working on this gene/protein

• Linc Sonenshein, Tufts University, Boston, MA, USA Homepage
• Jörg Stülke, University of Göttingen, Germany Homepage

References

Reviews

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Original publications

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